Detection of plasmid-mediated AmpC β-lactamases in Klebsiella pneumoniae clinical isolates from Bushehr province, Iran.

ABSTRACT:

Background and objectives

Plasmid-mediated AmpC producers are considered an increasing concern. The aim of this study was to investigate the prevalence of plasmid-mediated AmpC β-lactamases (pAmpCs) in Klebsiella pneumoniae isolates.

Materials and methods

A total of 228 clinical isolates of K. pneumoniae were collected in Bushehr province, Iran, from December 2017 to February 2019. Cefoxitin disks were applied for screening AmpC-producing isolates. Furthermore, 3 phenotypic confirmatory tests including combine disk test (CDT), double disk synergy test (DDST) and modified three dimensional test (M3DT) were used. Finally, the presence of pAmpC genes was tested by multiplex PCR.

Results

We identified 18 pAmpC-KP isolates among the 228 isolates (7.9%): 12 DH

SUBMITTER: Faghihi K 

PROVIDER: S-EPMC10336279 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature