Molecular identification of Anaplasma platys in cattle by nested PCR.

ABSTRACT:

Background and objectives

Anaplasmosis is a zoonotic disease caused by Gram-negative bacterium from Anaplasmataceae family. Anaplasma causes high economic losses worldwide. 16S rRNA analysis was used to diagnose Anaplasma platys in Cattle. Phylogenetic tree and estimation of evolutionary divergence between A. platys isolates were performed.

Materials and methods

A total of 60 blood samples were collected from a cattle farm in AL-Diwaniyah province. 16S rRNA gene was identified using nested PCR. Overall, 40% of cattle that were chosen to collect the blood were identified to be infected with A. platys.

Results

The results have shown presence of targeting partial region of 16S rRNA gene in 24 samples out of 60. Sequencing results of 10 samples have r

SUBMITTER: Al-Saadi M 

PROVIDER: S-EPMC10336285 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature