Mechanism of immune escape mediated by receptor tyrosine kinase KIT in thyroid cancer.

ABSTRACT:

Objective

Thyroid cancer (TC) is one of the fastest-growing malignant tumors. This study sought to explore the mechanism of immune escape mediated by receptor tyrosine kinase (KIT) in TC.

Methods

The expression microarray of TC was acquired through the GEO database, and the difference analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were carried out. KIT levels in TC cell lines (K1/SW579/BCPAP) and human normal thyroid cells were detected using reverse transcription quantitative polymerase chain reaction and western blot analysis. TC cells were transfected with overexpressed (oe)-KIT and CD8+ T cells were cocultured with SW579 cells. Subsequently, cell proliferation, migration, and invasion abilities, CD8+ T cell proliferation, cytokine levels

SUBMITTER: Luo Z 

PROVIDER: S-EPMC10336654 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature