Integration of gut microbiota and metabolomics for the hematopoiesis of Siwu paste on anemia rats.

ABSTRACT:

Background

To investigate the regulation mechanism of hematopoiesis of Siwu paste (SWP) in anemia rats, which is a classic Chinese prescription used for nourishing blood or blood deficiency over 1000 years.

Methods

Blood cell and biochemical analysis were used to evaluate the hematopoietic function of SWP in anemia rats. The intestinal microbial composition was analyzed with 16S rRNA gene sequencing, and the metabolites were profiled using UPLC-TripleTOF system nontargeting metabolomics.

Results

SWP can improve the levels of red blood cells, hemoglobin, platelet, hematocrit value, white blood cells, lymphocyte, EPO, TPO, and GM-CSF in anemia rats, and significantly change the microbial community and its metabolites. The correlation analysis of intestinal microbiota-he

SUBMITTER: Wan D 

PROVIDER: S-EPMC10336798 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature