The RNA m⁶A landscape of mouse oocytes and preimplantation embryos.

ABSTRACT: Despite the significance of N⁶-methyladenosine (m⁶A) in gene regulation, the requirement for large amounts of RNA has hindered m⁶A profiling in mammalian early embryos. Here we apply low-input methyl RNA immunoprecipitation and sequencing to map m⁶A in mouse oocytes and preimplantation embryos. We define the landscape of m⁶A during the maternal-to-zygotic transition, including stage-specifically expressed transcription factors essential for cell fate determination. Both the maternally inherited transcripts to be degraded post fertilization and the zygotically activated genes during zygotic genome activation are widely marked by m⁶A. In contrast to m⁶A-marked zygotic ally-activated genes, m⁶A-marked mate

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10337017 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature