PHF5A regulates the expression of the DOCK5 variant to promote HNSCC progression through p38 MAPK activation.

ABSTRACT:

Background

Previously, we identified an oncogenic splicing variant of DOCK5 in head and neck squamous cell carcinoma (HNSCC); however, the mechanism for the generation of this specific DOCK5 variant remains unknown. This study aims to explore the potential spliceosome genes involved in the production of the DOCK5 variant and validate its role in regulating the progression of HNSCC.

Methods

The differentially expressed spliceosome genes involved in the DOCK5 variant were analysed in The Cancer Genome Atlas (TCGA), and the correlation between the DOCK5 variant and the potential spliceosome gene PHF5A was verified by qRT-PCR. The expression of PHF5A was detected in HNSCC cells, TCGA data and a separate primary tumour cohort. The functional role of PHF5A was examined using CCK-8

SUBMITTER: Liu C 

PROVIDER: S-EPMC10337101 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature