Next-generation sequencing reveals mitogenome diversity in plasma extracellular vesicles from colorectal cancer patients.

ABSTRACT:

Background

Recent reports have demonstrated that the entire mitochondrial genome can be secreted in extracellular vesicles (EVs), but the biological attributes of this cell-free mitochondrial DNA (mtDNA) remain insufficiently understood. We used next-generation sequencing to compare plasma EV-derived mtDNA to that of whole blood (WB), peripheral blood mononuclear cells (PBMCs), and formalin-fixed paraffin-embedded (FFPE) tumor tissue from eight rectal cancer patients and WB and fresh-frozen (FF) tumor tissue from eight colon cancer patients.

Methods

Total DNA was isolated before the mtDNA was enriched by PCR with either two primer sets generating two long products or multiple primer sets (for the FFPE tumors), prior to the sequencing. mtDNA diversity was assessed as the tota

SUBMITTER: Bjornetro T 

PROVIDER: S-EPMC10337118 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature