Inconsistencies in variable reporting and methods in larval zebrafish behavioral assays.

ABSTRACT: New approaches in developmental neurotoxicity (DNT) screening are needed due to the tens of thousands of chemicals requiring hazard assessments. Zebrafish (Danio rerio) are an alternative vertebrate model for DNT testing, but without a standardized protocol for larval behavioral assays, comparison of results among laboratories is challenging. To evaluate the congruence of protocols across laboratories, we conducted a literature review of DNT studies focusing on larval zebrafish behavior assays and cataloged experimental design consistencies. Our review focused on 51 unique method variables in publications where chemical exposure occurred in early development and subsequent larval locomotor evaluation focused on assays that included a light/dark photoperiod transition. We initially identifi

SUBMITTER: Hill BN 

PROVIDER: S-EPMC10337341 | biostudies-literature | 2023 Mar-Apr

REPOSITORIES: biostudies-literature