CD63/81 Small Extracellular Vesicles in the Aqueous Humor are Retinoblastoma Associated.

ABSTRACT:

Purpose

Although biopsy is contraindicated in retinoblastoma (RB), the aqueous humor (AH) is a robust liquid biopsy source of molecular tumor information, facilitating biomarker discovery. Small extracellular vesicles (sEVs), promising biomarker candidates across multiple cancers, were recently identified in RB AH, but relationships between sEVs and RB clinical features are unknown.

Methods

We analyzed sEVs in 37 AH samples from 18 RB eyes of varying International Intraocular Retinoblastoma Classification (IIRC) groups and explored clinical correlations. Ten samples were collected at diagnosis (DX) and 27 during treatment (Tx). Unprocessed AH underwent Single Particle-Interferometric Reflectance Imaging Sensor (SP-IRIS) analysis for fluorescent particle count and tetraspanin

SUBMITTER: Pike S 

PROVIDER: S-EPMC10337798 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature