Insights into Nitrosoalkane Binding to Myoglobin Provided by Crystallography of Wild-Type and Distal Pocket Mutant Derivatives.

ABSTRACT: Nitrosoalkanes (R-N═O; R = alkyl) are biological intermediates that form from the oxidative metabolism of various amine (RNH₂) drugs or from the reduction of nitroorganics (RNO₂). RNO compounds bind to and inhibit various heme proteins. However, structural information on the resulting Fe-RNO moieties remains limited. We report the preparation of ferrous wild-type and H64A sw Mb^II-RNO derivatives (λ_max 424 nm; R = Me, Et, Pr, iPr) from the reactions of Mb^III-H₂O with dithionite and nitroalkanes. The apparent extent of formation of the wt Mb derivatives followed the order MeNO > EtNO > PrNO > iPrNO, whereas the order was the opposite for the H64A derivatives. Ferricyanide oxidation of the Mb^II-RNO deri

SUBMITTER: Herrera VE 

PROVIDER: S-EPMC10338068 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature