Phenytoin regulates osteogenic differentiation of human bone marrow stem cells by PI3K/Akt pathway.

ABSTRACT:

Background

We mainly studied the mechanism by which phenytoin promotes osteogenic differentiation of human jawbone marrow stem cells.

Methods

Bone marrow stem cells were extracted from jaw bone tissue debris obtained from 5 subjects undergoing implant restoration. Osteogenic and adipogenic experiments proved cells stemness, and the expression of ALP, RUNX2, and OSX were detected by qPCR and Western blot. High-throughput sequencing was used to extract differentially expressed genes, the network database predicted phenytoin drug targets, GO and KEGG enrichment combined with PPI network diagram to analyze the osteogenesis mechanism.

Results

Calcium nodules and lipid droplet formation were observed in osteogenic and adipogenic experiments. The concentration of phenytoin w

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10338146 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature