Histone modifications regulate pioneer transcription factor cooperativity.

ABSTRACT: Pioneer transcription factors have the ability to access DNA in compacted chromatin¹. Multiple transcription factors can bind together to a regulatory element in a cooperative way, and cooperation between the pioneer transcription factors OCT4 (also known as POU5F1) and SOX2 is important for pluripotency and reprogramming^2-4. However, the molecular mechanisms by which pioneer transcription factors function and cooperate on chromatin remain unclear. Here we present cryo-electron microscopy structures of human OCT4 bound to a nucleosome containing human LIN28B or nMATN1 DNA sequences, both of which bear multiple binding sites for OCT4. Our structural and biochemistry data reveal that binding of OCT4 induces changes to the nucleosome structure, repositions the nucleosoma

SUBMITTER: Sinha KK 

PROVIDER: S-EPMC10338341 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature