OrganoidChip facilitates hydrogel-free immobilization for fast and blur-free imaging of organoids.

ABSTRACT: Organoids are three-dimensional structures of self-assembled cell aggregates that mimic anatomical features of in vivo organs and can serve as in vitro miniaturized organ models for drug testing. The most efficient way of studying drug toxicity and efficacy requires high-resolution imaging of a large number of organoids acquired in the least amount of time. Currently missing are suitable platforms capable of fast-paced high-content imaging of organoids. To address this knowledge gap, we present the OrganoidChip, a microfluidic imaging platform that incorporates a unique design to immobilize organoids for endpoint, fast imaging. The chip contains six parallel trapping areas, each having a staging and immobilization chamber, that receives organoids transferred from their native culture plate

SUBMITTER: Moshksayan K 

PROVIDER: S-EPMC10338466 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature