Pilot study investigating BP-180 in extracellular vesicles derived from blister fluid of bullous pemphigoid patients.

ABSTRACT: Bullous pemphigoid (BP) is an autoimmune blistering disease that targets the haemidesmosomal proteins, mainly BP180. Extracellular vesicles (EVs) have been demonstrated to carry tissue-specific autoantigens in the setting of autoimmune diseases and transplant organ rejection; this phenomenon was demonstrated to have pathogenic implications in autoimmune diseases and to correlate with transplant rejection severity. The purpose of this study was to identify the presence of BP targeted autoantigens in blister fluid derived EVs. We isolated, by size exclusion chromatography, EVs derived from blisters of BP-patients and from suction blisters of healthy donors. EV characterization was performed by flow cytometry and nanoparticle tracking analysis. Western blot analysis was used to investigate th

SUBMITTER: Gasparini G 

PROVIDER: S-EPMC10338600 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature