NDRG1 facilitates self-renewal of liver cancer stem cells by preventing EpCAM ubiquitination.

ABSTRACT:

Background

Portal vein tumour thrombus (PVTT) is the main pathway of HCC intrahepatic metastasis and is responsible for the poor prognosis of patients with HCC. However, the molecular mechanisms underlying PVTT vascular metastases have not been fully elucidated.

Methods

NDRG1 expression was assessed by immunohistochemistry and immunoblotting in clinical specimens obtained from curative surgery. The functional relevance of NDRG1 was evaluated using sphere formation and animal models of tumorigenicity and metastasis. The relationship between NDRG1 and EpCAM was explored using molecular biological techniques.

Results

NDRG1 protein was upregulated in HCC samples compared to non-tumorous tissues. Furthermore, NDRG1 expression was enhanced in the PVTT samples. Our functiona

SUBMITTER: Cheng Q 

PROVIDER: S-EPMC10338678 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature