Whole genome analysis and antimicrobial resistance of Neisseria gonorrhoeae isolates from Ghana.

ABSTRACT:

Introduction

Gonorrhoea is a major public health concern. With the global emergence and spread of resistance to last-line antibiotic treatment options, gonorrhoea threatens to be untreatable in the future. Therefore, this study performed whole genome characterization of Neisseria gonorrhoeae collected in Ghana to identify lineages of circulating strains as well as their phenotypic and genotypic antimicrobial resistance (AMR) profiles.

Methods

Whole genome sequencing (WGS) was performed on 56 isolates using both the Oxford Nanopore MinION and Illumina MiSeq sequencing platforms. The Comprehensive Antimicrobial Resistance Database (CARD) and PUBMLST.org/neisseria databases were used to catalogue chromosomal and plasmid genes implicated in AMR. The core genome multi-locu

SUBMITTER: Agbodzi B 

PROVIDER: S-EPMC10339232 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature