Cancer stem cells promote lymph nodes metastasis of breast cancer by reprogramming tumor microenvironment.

ABSTRACT: Breast cancer progression and metastasis are governed by a complex interplay within the tumor immune microenvironment (TIME), involving numerous cell types. Lymph node metastasis (LNM) is a key prognostic marker associated with distant organ metastasis and reduced patient survival, but the mechanisms underlying its promotion by breast cancer stem cells (CSCs) remain unclear. Our study sought to unravel how CSCs reprogram TIME to facilitate LNM. Utilizing single-cell RNA sequencing, we profiled TIME in primary cancer and corresponding metastatic lymph node samples from patients at our institution. To verify the derived data, we cultured CSCs and performed validation assays employing flow cytometry and CyTOF. Our analysis revealed distinct differences in cellular infiltration patterns betwee

SUBMITTER: Li L 

PROVIDER: S-EPMC10339262 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature