Conserved Evolution of MHC Supertypes among Japanese Frogs Suggests Selection for Bd Resistance.

ABSTRACT: The chytrid fungus Batrachochytrium dendrobatidis (Bd) is a major threat to amphibians, yet there are no reports of major disease impacts in East Asian frogs. Genetic variation of the major histocompatibility complex (MHC) has been associated with resistance to Bd in frogs from East Asia and worldwide. Using transcriptomic data collated from 11 Japanese frog species (one individual per species), we isolated MHC class I and IIb sequences and validated using molecular cloning. We then compared MHC from Japanese frogs and other species worldwide, with varying Bd susceptibility. Supertyping analysis, which groups MHC alleles based on physicochemical properties of peptide binding sites, identified that all examined East Asian frogs contained at least one MHC-IIb allele belonging to super

SUBMITTER: Lau Q 

PROVIDER: S-EPMC10340028 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature