The Effect of Methodological Considerations on the Construction of Gene-Based Plant Pan-genomes.

ABSTRACT: Pan-genomics is an emerging approach for studying the genetic diversity within plant populations. In contrast to common resequencing studies that compare whole genome sequencing data with a single reference genome, the construction of a pan-genome (PG) involves the direct comparison of multiple genomes to one another, thereby enabling the detection of genomic sequences and genes not present in the reference, as well as the analysis of gene content diversity. Although multiple studies describing PGs of various plant species have been published in recent years, a better understanding regarding the effect of the computational procedures used for PG construction could guide researchers in making more informed methodological decisions. Here, we examine the effect of several key methodological f

SUBMITTER: Glick L 

PROVIDER: S-EPMC10340445 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature