Isotopic Tracing of Nucleotide Sugar Metabolism in Human Pluripotent Stem Cells.

ABSTRACT: Metabolism not only produces energy necessary for the cell but is also a key regulator of several cellular functions, including pluripotency and self-renewal. Nucleotide sugars (NSs) are activated sugars that link glucose metabolism with cellular functions via protein N-glycosylation and O-GlcNAcylation. Thus, understanding how different metabolic pathways converge in the synthesis of NSs is critical to explore new opportunities for metabolic interference and modulation of stem cell functions. Tracer-based metabolomics is suited for this challenge, however chemically-defined, customizable media for stem cell culture in which nutrients can be replaced with isotopically labeled analogs are scarcely available. Here, we established a customizable flux-conditioned E8 (FC-E8) medium that enables

SUBMITTER: Conte F 

PROVIDER: S-EPMC10340731 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature