Dataset Information

Biosynthesis and Characterization of Silver Nanoparticles Produced by Parachlorella kessleri and Cyclotella spp., and the Evaluation of Their Antibacterial Activity.

ABSTRACT: Green synthesis is one of the fastest and best ways for ecofriendly nanoparticle synthesis. This study aims to investigate the use of the green microalgae Parachlorella kesseleri and Cyclotella spp. for the biological synthesis of silver nanoparticles (AgNPs). This work focuses on optimizing various parameters necessary for the production and stability of AgNPs. The nanoparticle formation was confirmed by UV-Visible analysis, which revealed the surface plasmon resonance band at 420 nm. The characterization of the AgNPs was performed using UV-visible spectroscopy, X-ray diffraction (XRD), scanning electron microscopy coupled with energy dispersive X-ray microanalysis (SEM-EDS), Fourier transform infrared spectroscopy (FTIR), and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The antimicrobial properties of these bioactive AgNPs were also tested, showing excellent antibacterial activity against six bacterial strains, Escherichia coli, multidrug-resistant Escherichia coli, Bacillus clausii, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhi. The biosynthesis of AgNPs from living cultures of microalgae has remarkable antibacterial properties. Other studies are underway in our laboratory to clarify the mechanism of the biosynthesis of these nanoparticles, and their action on bacteria.

SUBMITTER: El Ouardy K

PROVIDER: S-EPMC10341437 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Biosynthesis and Characterization of Silver Nanoparticles Produced by Parachlorella kessleri and Cyclotella spp., and the Evaluation of Their Antibacterial Activity.

El Ouardy Khadija K Lbouhmadi Rkia R Attaoui Hind H Mouzaki Mustapha M Mouine Hanane H Lemkhente Zohra Z Mir Youssef Y

International journal of molecular sciences 20230625 13

Green synthesis is one of the fastest and best ways for ecofriendly nanoparticle synthesis. This study aims to investigate the use of the green microalgae Parachlorella kesseleri and Cyclotella spp. for the biological synthesis of silver nanoparticles (AgNPs). This work focuses on optimizing various parameters necessary for the production and stability of AgNPs. The nanoparticle formation was confirmed by UV-Visible analysis, which revealed the surface plasmon resonance band at 420 ...[more]

PMID: 37445777

Similar Datasets

Project description:Attached culture allows high biomass productivity and is a promising biomass cultivating system because neither a huge facility area nor a large volume of culture medium are needed. This study investigates photosynthetic and transcriptomic behaviors in Parachlorella kessleri cells on a solid surface after their transfer from liquid culture to elucidate the physiological and gene-expression regulatory mechanisms that underlie their vigorous proliferation. The chlorophyll content shows a decrease at 12 h after the transfer; however, it has fully recovered at 24 h, suggesting temporary decreases in the amounts of light harvesting complexes. On PAM analysis, it is demonstrated that the effective quantum yield of PSII decreases at 0 h right after the transfer, followed by its recovery in the next 24 h. A similar changing pattern is observed for the photochemical quenching, with the PSII maximum quantum yield remaining at an almost unaltered level. Non-photochemical quenching was increased at both 0 h and 12 h after the transfer. These observations suggest that electron transfer downstream of PSII but not PSII itself is only temporarily damaged in solid-surface cells just after the transfer, with light energy in excess being dissipated as heat for PSII protection. It thus seems that the photosynthetic machinery acclimates to high-light and/or dehydration stresses through its temporal size-down and functional regulation that start right after the transfer. Meanwhile, transcriptomic analysis by RNA-Seq demonstrates temporary upregulation at 12 h after the transfer as to the expression levels of many genes for photosynthesis, amino acid synthesis, general stress response, and ribosomal subunit proteins. These findings suggest that cells transferred to a solid surface become stressed immediately after transfer but can recover their high photosynthetic activity through adaptation of photosynthetic machinery and metabolic flow as well as induction of general stress response mechanisms within 24 h.

Dataset Information

Biosynthesis and Characterization of Silver Nanoparticles Produced by Parachlorella kessleri and Cyclotella spp., and the Evaluation of Their Antibacterial Activity.

Publications

Biosynthesis and Characterization of Silver Nanoparticles Produced by <i>Parachlorella kessleri</i> and <i>Cyclotella</i> spp., and the Evaluation of Their Antibacterial Activity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets