A Novel Strategy for Screening Tumor-Specific Variable Domain of Heavy-Chain Antibodies.

ABSTRACT: The properties of the variable domain of heavy-chain (VHH) antibodies are particularly relevant in cancer therapy. To isolate tumor cell-specific VHH antibodies, VHH phage libraries were constructed from multiple tumor cells. After enriching the libraries against particular tumor cell lines, a next-generation sequencer was used to screen the pooled phages of each library for potential antibody candidates. Based on high amplification folds, 50 sequences from each library were used to construct phylogenetic trees. Several clusters with identical CDR3 were observed. Groups X, Y, and Z were assigned as common sequences among the different trees. These identical groups over the trees were considered to be cross-reactive antibodies. To obtain monoclonal antibodies, we assembled 200 sequences (to

SUBMITTER: Rafique A 

PROVIDER: S-EPMC10341490 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature