Double-Labeling Method for Visualization and Quantification of Membrane-Associated Proteins in <i>Lactococcus lactis</i>.

ABSTRACT: Lactococcus lactis displaying recombinant proteins on its surface can be used as a potential drug delivery vector in prophylactic medication and therapeutic treatments for many diseases. These applications enable live-cell mucosal and oral administration, providing painless, needle-free solutions and triggering robust immune response at the site of pathogen entry. Immunization requires quantitative control of antigens and, ideally, a complete understanding of the bacterial processing mechanism applied to the target proteins. In this study, we propose a double-labeling method based on a conjugated dye specific for a recombinantly introduced polyhistidine tag (to visualize surface-exposed proteins) and a membrane-permeable dye specific for a tetra-cysteine tag (to visualize cytoplasmi

SUBMITTER: Hoang MN 

PROVIDER: S-EPMC10341821 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature