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ABF1 Positively Regulates Rice Chilling Tolerance via Inducing Trehalose Biosynthesis.

ABSTRACT: Chilling stress seriously limits grain yield and quality worldwide. However, the genes and the underlying mechanisms that respond to chilling stress remain elusive. This study identified ABF1, a cold-induced transcription factor of the bZIP family. Disruption of ABF1 impaired chilling tolerance with increased ion leakage and reduced proline contents, while ABF1 over-expression lines exhibited the opposite tendency, suggesting that ABF1 positively regulated chilling tolerance in rice. Moreover, SnRK2 protein kinase SAPK10 could phosphorylate ABF1, and strengthen the DNA-binding ability of ABF1 to the G-box cis-element of the promoter of TPS2, a positive regulator of trehalose biosynthesis, consequently elevating the TPS2 transcription and the endogenous trehalose contents. Meanwhile, applying exogenous trehalose enhanced the chilling tolerance of abf1 mutant lines. In summary, this study provides a novel pathway 'SAPK10-ABF1-TPS2' involved in rice chilling tolerance through regulating trehalose homeostasis.

SUBMITTER: Shu Y

PROVIDER: S-EPMC10342119 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature

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ABF1 Positively Regulates Rice Chilling Tolerance via Inducing Trehalose Biosynthesis.

Shu Yazhou Y Zhang Wensheng W Tang Liqun L Li Zhiyong Z Liu Xinyong X Liu Xixi X Liu Wanning W Li Guanghao G Ying Jiezheng J Huang Jie J Tong Xiaohong X Hu Honghong H Zhang Jian J Wang Yifeng Y

International journal of molecular sciences 20230704 13

Chilling stress seriously limits grain yield and quality worldwide. However, the genes and the underlying mechanisms that respond to chilling stress remain elusive. This study identified ABF1, a cold-induced transcription factor of the bZIP family. Disruption of <i>ABF1</i> impaired chilling tolerance with increased ion leakage and reduced proline contents, while <i>ABF1</i> over-expression lines exhibited the opposite tendency, suggesting that ABF1 positively regulated chilling tolerance in ric ...[more]

PMID: 37446259

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Project description:The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of OsPIN9 leads to altered plant architecture and chilling tolerance in rice. The expression profile analysis indicated that OsPIN9 was gradually suppressed by chilling stress. The shoot height and adventitious root number of OsPIN9-overexpressing (OE) plants were significantly reduced at the seedling stage. The roots of OE plants were more tolerant to N-1-naphthylphthalamic acid (NPA) treatment than WT plants, indicating the disturbance of auxin homeostasis in OE lines. The chilling tolerance assay showed that the survival rate of OE plants was markedly lower than that of wild-type (WT) plants. Consistently, more dead cells, increased electrolyte leakage, and increased malondialdehyde (MDA) content were observed in OE plants compared to those in WT plants under chilling conditions. Notably, OE plants accumulated more hydrogen peroxide (H2O2) and less superoxide anion radicals (O2-) than WT plants under chilling conditions. In contrast, catalase (CAT) and superoxide dismutase (SOD) activities in OE lines decreased significantly compared to those in WT plants at the early chilling stage, implying that the impaired chilling tolerance of transgenic plants is probably attributed to the sharp induction of H2O2 and the delayed induction of antioxidant enzyme activities at this stage. In addition, several OsRboh genes, which play a crucial role in ROS production under abiotic stress, showed an obvious increase after chilling stress in OE plants compared to that in WT plants, which probably at least in part contributes to the production of ROS under chilling stress in OE plants. Together, our results reveal that OsPIN9 plays a vital role in regulating plant architecture and, more importantly, is involved in regulating rice chilling tolerance by influencing auxin and ROS homeostasis.

Dataset Information

ABF1 Positively Regulates Rice Chilling Tolerance via Inducing Trehalose Biosynthesis.

Publications

ABF1 Positively Regulates Rice Chilling Tolerance via Inducing Trehalose Biosynthesis.

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