The Toll-like Receptor 7-Mediated Ro52 Antigen-Presenting Pathway in the Salivary Gland Epithelial Cells of Sjogren's Syndrome.

ABSTRACT:

Objective

To investigate whether stimulation with toll-like receptor (TLR) 7 leads to pathways that proceed to tripartite motif-containing protein 21 (TRIM21) or Ro52/SS-A antigen presentation through major histocompatibility complex (MHC) class I in salivary gland epithelial cells (SGECs) from Sjögren's syndrome (SS) patients.

Design and methods

Cultured SGECs from SS patients were stimulated with TLR7 agonist, loxoribine, and interferon-β. Cell lysates immunoprecipitated by anti-MHC class I antibody were analyzed by Western blotting. The immunofluorescence of salivary gland tissue from SS and non-SS subjects and cultured TLR7-stimulated SGECs was examined.

Results

Significantly increased MHC class I expression was observed in SS patients' ducts versus non-SS ducts;

SUBMITTER: Nishihata SY 

PROVIDER: S-EPMC10342814 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature