Quantification of the capacity of vibrio fischeri to establish symbiosis with Euprymna scolopes.

ABSTRACT: Most animals establish long-term symbiotic associations with bacteria that are critical for normal host physiology. The symbiosis that forms between the Hawaiian squid Euprymna scolopes and the bioluminescent bacterium Vibrio fischeri serves as an important model system for investigating the molecular mechanisms that promote animal-bacterial symbioses. E. scolopes hatch from their eggs uncolonized, which has led to the development of squid-colonization assays that are based on introducing culture-grown V. fischeri cells to freshly hatched juvenile squid. Recent studies have revealed that strains often exhibit large differences in how they establish symbiosis. Therefore, we sought to develop a simplified and reproducible protocol that permits researchers to determine appropriate inoculum le

SUBMITTER: Donnelly AR 

PROVIDER: S-EPMC10343157 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature