Active Zone Trafficking of CaV2/UNC-2 Channels Is Independent of β/CCB-1 and α2δ/UNC-36 Subunits.

ABSTRACT: The CaV2 voltage-gated calcium channel is the major conduit of calcium ions necessary for neurotransmitter release at presynaptic active zones (AZs). The CaV2 channel is a multimeric complex that consists of a pore-forming α₁ subunit and two auxiliary β and α₂δ subunits. Although auxiliary subunits are critical for channel function, whether they are required for α₁ trafficking is unresolved. Using endogenously fluorescent protein-tagged CaV2 channel subunits in Caenorhabditis elegans, we show that UNC-2/α₁ localizes to AZs even in the absence of CCB-1/β or UNC-36/α₂δ, albeit at low levels. When UNC-2 is manipulated to be trapped in the endoplasmic reticulum (ER), CCB-1 and UNC-36 fail to colocalize with UNC-2 in the ER, indicati

SUBMITTER: Oh KH 

PROVIDER: S-EPMC10343168 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature