The quest for the holy grail: overcoming challenges in expanding human hematopoietic stem cells for clinical use.

ABSTRACT: Hematopoietic stem cell (HSC) transplantation has been the golden standard for many hematological disorders. However, the number of HSCs obtained from several sources, including umbilical cord blood (UCB), often is insufficient for transplantation. For decades, maintaining or even expanding HSCs for therapeutic purposes has been a "holy grail" in stem cell biology. Different methods have been proposed to improve the efficiency of cell expansion and enhance homing potential such as co-culture with stromal cells or treatment with specific agents. Recent progress has shown that this is starting to become feasible using serum-free and well-defined media. Some of these protocols to expand HSCs along with genetic modification have been successfully applied in clinical trials and some others are

SUBMITTER: Bastani S 

PROVIDER: S-EPMC10345135 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature