Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers.

ABSTRACT: Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.

SUBMITTER: Liu X 

PROVIDER: S-EPMC10345194 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature