Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant in Medicago truncatula.

ABSTRACT: Medicago truncatula has been selected as one of the model legume species for gene functional studies. To elucidate the functions of the very large number of genes present in plant genomes, genetic mutant resources are very useful and necessary tools. Fast Neutron (FN) mutagenesis is effective in inducing deletion mutations in genomes of diverse species. Through this method, we have generated a large mutant resource in M. truncatula. This mutant resources have been used to screen for different mutant using a forward genetics methods. We have isolated and identified a large amount of symbiotic nitrogen fixation (SNF) deficiency mutants. Here, we describe the detail procedures that are being used to characterize symbiotic mutants in M. truncatula. In recent years, whole g

SUBMITTER: Shen Y 

PROVIDER: S-EPMC10345209 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature