PQBP1 regulates the cellular inflammation induced by avian reovirus and interacts with the viral p17 protein.

ABSTRACT: Avian reovirus (ARV) can commonly infect a flock and cause immunosuppressive diseases in poultry. The nonstructural protein p17 is involved in viral replication, and significant progress has been made in showing its ability to regulate cellular signaling pathways. In our previous study, to further investigate the effect of ARV p17 protein on viral replication, the host protein polyglu-tamine binding protein 1 (PQBP1) was identified to interact with p17 by a yeast two-hybrid system. In the current study, the interaction between PQBP1 and p17 protein was further confirmed by laser confocal microscopy and coimmunoprecipitation assays. In addition, the N-terminal WWD of PQBP1 was found to mediate the process of binding to the p17 protein. Interestingly, we found that ARV infection significantl

SUBMITTER: Zhang C 

PROVIDER: S-EPMC10345743 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature