Firefly luciferin methyl ester illuminates the activity of multiple serine hydrolases.

ABSTRACT: Firefly luciferin methyl ester is hydrolyzed by monoacylglycerol lipase MAGL, amidase FAAH, poorly-characterized hydrolase ABHD11, and hydrolases known for S-depalmitoylation (LYPLA1/2), not just esterase CES1. This enables activity-based bioluminescent assays for serine hydrolases and suggests that the 'esterase activity' responsible for hydrolyzing ester prodrugs is more diverse than previously supposed.

SUBMITTER: Mohammad I 

PROVIDER: S-EPMC10347678 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature