Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids.

ABSTRACT:

Background

Prime editing (PE) is the most recent gene editing technology able to introduce targeted alterations to the genome, including single base pair changes, small insertions, and deletions. Several improvements to the PE machinery have been made in the past few years, and these have been tested in a range of model systems including immortalized cell lines, stem cells, and animal models. While double nicking RNA (dncRNA) PE systems PE3 and PE5 currently show the highest editing rates, they come with reduced accuracy as undesired indels or SNVs arise at edited loci. Here, we aimed to improve single ncRNA (sncRNA) systems PE2 and PE4max by generating novel all-in-one (pAIO) plasmids driven by an EF-1α promoter, which is especially suitable for human-induced pluripotent stem cell

SUBMITTER: Dirkx N 

PROVIDER: S-EPMC10347817 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature