Ensemble Force Spectroscopy of a G-Quadruplex Cluster on a Single-Molecule Platform.

ABSTRACT: Single-molecule methods offer high sensitivities with precisions superior to bulk assays. However, these methods are low in throughput and cannot repetitively interrogate the same cluster of molecular units. In this work, we investigate a tandem array of G-quadruplexes on a single-molecule DNA template with a throughput of at least two orders of magnitude higher than single-molecule force spectroscopy. During mechanical unfolding by optical tweezers, the array of G-quadruplexes experiences identical force, temperature, and ionic conditions, which not only reduce environmental noise but also render unfolding transitions indistinguishable among individual G-quadruplexes. The resultant ensemble behaviors are analyzed by scanning force diagrams, which reveals accurate F_1/2 va

SUBMITTER: Pokhrel P 

PROVIDER: S-EPMC10347895 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature