Force-Dependent Structural Changes of Filamin C Rod Domains Regulated by Filamin C Dimer.

ABSTRACT: Filamin C (FLNC), a large dimeric actin-binding protein in muscle cells, plays a critical role in transmitting force in the cytoskeleton and that between membrane receptors and the cytoskeleton. It performs crucial mechanosensing and downstream mechanotransduction functions via force-dependent interactions with signaling proteins. Mutations in FLNC have been linked to muscle and heart diseases. The mechanical responses of the force-bearing elements in FLNC have not been determined. This study investigated the mechanical responses of FLNC domains and their dimerization interface using magnetic tweezers. Results showed high stability of the N-terminal domains in the rod-1 segment but significant changes in the rod-2 domains in response to forces of a few piconewtons (pN). The dimerization in

SUBMITTER: Deng Y 

PROVIDER: S-EPMC10348313 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature