In-droplet hydrogen-deuterium exchange to examine protein/peptide solution conformer heterogeneity.

ABSTRACT:

Rationale

Many different structure analysis techniques are not capable of probing the heterogeneity of solution conformations. Here, we examine the ability of in-droplet hydrogen-deuterium exchange (HDX) to directly probe solution conformer heterogeneity of a protein with mass spectrometry (MS) detection.

Methods

Two vibrating capillary vibrating sharp-edge spray ionization (cVSSI) devices have been arranged such that they generate microdroplet plumes of the analyte and D₂ O reagent, which coalesce to form reaction droplets where HDX takes place in the solution environment. The native HDX-MS setup has been first explored for two model peptides that have distinct structural compositions in solution. The effectiveness of the multidevice cVSSI-HDX in illustrating str

SUBMITTER: Sharif D 

PROVIDER: S-EPMC10348485 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature