Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation.

ABSTRACT: During mammalian development, the left and right ventricles arise from early populations of cardiac progenitors known as the first and second heart fields, respectively. While these populations have been extensively studied in non-human model systems, their identification and study in vivo human tissues have been limited due to the ethical and technical limitations of accessing gastrulation-stage human embryos. Human-induced pluripotent stem cells (hiPSCs) present an exciting alternative for modeling early human embryogenesis due to their well-established ability to differentiate into all embryonic germ layers. Here, we describe the development of a TBX5/MYL2 lineage tracing reporter system that allows for the identification of FHF- progenitors and their descendants including left ventricu

SUBMITTER: Galdos FX 

PROVIDER: S-EPMC10348749 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature