Unanticipated broad phylogeny of BEN DNA-binding domains revealed by structural homology searches.

ABSTRACT: Organization of protein sequences into domain families is a foundation for cataloging and investigating protein functions. However, long-standing strategies based on primary amino acid sequences are blind to the possibility that proteins with dissimilar sequences could have comparable tertiary structures. Building on our recent findings that in silico structural predictions of BEN family DNA-binding domains closely resemble their experimentally determined crystal structures, we exploited the AlphaFold2 database for comprehensive identification of BEN domains. Indeed, we identified numerous novel BEN domains, including members of new subfamilies. For example, while no BEN domain factors had previously been annotated in C. elegans, this species actually encodes multiple BEN proteins. These i

SUBMITTER: Pan A 

PROVIDER: S-EPMC10348805 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature