Continuous vs. discontinuous purification of isolated human islets: functional and morphological comparison.

ABSTRACT:

Background

The COBE 2991 cell processor, commonly used for pancreatic islet isolation, is no longer distributed in Europe, leading to a search for alternative purification procedures with equivalent efficacy. The aim of this study was to evaluate the efficacy of an alternative method based on the discontinuous purification of islets.

Methods

The conventional isolation procedure using a standard continuous islet purification with COBE 2991 of n = 4 human pancreas was compared to n = 8 procedures using a discontinuous purification with a "bottle" method from donors of similar characteristics. Islet equivalents, purity, and dynamic glucose-stimulated insulin secretion were evaluated.

Results

A similar islet yield was obtained using continuous vs. discontinu

SUBMITTER: Buemi A 

PROVIDER: S-EPMC10348810 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature