Nanoscale bond-selective imaging by computational fusion of atomic force microscopy and coherent anti-Stokes Raman scattering microscopy.

ABSTRACT: Vibrational microscopy based on coherent Raman scattering is a powerful tool for high-speed chemical imaging, but its lateral resolution is bound to the optical diffraction limit. On the other hand, atomic force microscopy (AFM) provides nano-scale spatial resolution, yet with lower chemical specificity. In this study, we leverage a computational approach called pan-sharpening to merge AFM topography images and coherent anti-Stokes Raman scattering (CARS) images. The hybrid system combines the advantages of both modalities, providing informative chemical mapping with ∼20 nm spatial resolution. CARS and AFM images were sequentially acquired on a single multimodal platform, which facilitates image co-localization. Our image fusion approach allowed for discerning merged neighboring features p

SUBMITTER: Wang L 

PROVIDER: S-EPMC10349369 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature