Single-cell analysis of white adipose tissue reveals the tumor-promoting adipocyte subtypes.

ABSTRACT:

Background

The tumor-adipose microenvironment (TAME) is characterized by the enrichment of adipocytes, and is considered a special ecosystem that supports cancer progression. However, the heterogeneity and diversity of adipocytes in TAME remains poorly understood.

Methods

We conducted a single-cell RNA sequencing analysis of adipocytes in mouse and human white adipose tissue (WAT). We analyzed several adipocyte subtypes to evaluate their relationship and potential as prognostic factors for overall survival (OS). The potential drugs are screened by using bioinformatics methods. The tumor-promoting effects of a typical adipocyte subtype in breast cancer are validated by performing in vitro functional assays and immunohistochemistry (IHC) in clinical samples.

Results

We

SUBMITTER: Liu SQ 

PROVIDER: S-EPMC10349475 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature