Comparison of osteoclast differentiation protocols from human induced pluripotent stem cells of different tissue origins.

ABSTRACT:

Background

Ever since their discovery, induced pluripotent stem cells (iPSCs) have been extensively differentiated into a large variety of cell types. However, a limited amount of work has been dedicated to differentiating iPSCs into osteoclasts. While several differentiation protocols have been published, it remains unclear which protocols or differentiation methods are preferrable regarding the differentiation of osteoclasts.

Methods

In this study we compare the osteoclastogenesis capacity of a peripheral blood mononuclear cell (PBMC)-derived iPSC line to a fibroblast-derived iPSC line in conjunction with either embryoid body-based or monolayer-based differentiation strategies. Both cell lines and differentiation protocols were investigated regarding their ability to gener

SUBMITTER: Blumke A 

PROVIDER: S-EPMC10350192 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature