Structure and dynamics of endogenous protein complexes in human heart tissue captured by native nanoproteomics.

ABSTRACT: Protein complexes are highly dynamic entities that display substantial diversity in their assembly, post-translational modifications, and non-covalent interactions, allowing them to play critical roles in various biological processes. The heterogeneity, dynamic nature, and low abundance of protein complexes in their native states present tremendous challenges to study using conventional structural biology techniques. Here we develop a "native nanoproteomics" strategy for the native enrichment and subsequent native top-down mass spectrometry (nTDMS) of low-abundance protein complexes. Specifically, we demonstrate the first comprehensive characterization of the structure and dynamics of cardiac troponin (cTn) complexes directly from human heart tissue. The endogenous cTn complex is effective

SUBMITTER: Chapman EA 

PROVIDER: S-EPMC10350235 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature