Transplantation of adipose tissue-derived microvascular fragments promotes therapy of critical limb ischemia.

ABSTRACT:

Background

Adipose tissue-derived microvascular fragments are functional vessel segments derived from arterioles, capillaries, and veins. Microvascular fragments can be used as vascularization units in regenerative medicine and tissue engineering containing microvascular networks. However, the in vivo therapeutic and vascularization properties of human microvascular fragments have not been investigated.

Methods

In this study, we isolated microvascular fragments, stromal vascular fractions, and mesenchymal stem cells from human lipoaspirate and studied their therapeutic efficacy and in vivo vasculogenic activity in a murine model of hindlimb ischemia. In addition, in vivo angiogenic activity and engraftment of microvascular fragments into blood vessels were measured using Mat

SUBMITTER: Park GT 

PROVIDER: S-EPMC10350266 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature