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Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light.

ABSTRACT: A light-activated genome editing platform based on the release of enzymes from a plasmonic nanoparticle carrier when exposed to biocompatible near-infrared light pulses is described. The platform relies on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of copper which is attached to double-stranded nucleic acids self-assembled on the gold nanoparticle surface. A protein fusion of the Cre recombinase containing a TAT internalization peptide sequence to achieve endosomal localization is also employed. High-resolution gene knock-in of a red fluorescent reporter is observed using a commercial two-photon microscope. High-throughput irradiation is described to generate useful quantities of edited cells.

SUBMITTER: Morales DP

PROVIDER: S-EPMC10350682 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Light-Triggered Genome Editing: Cre Recombinase Mediated Gene Editing with Near-Infrared Light.

Morales Demosthenes P DP Morgan Erin N EN McAdams Meghan M Chron Amanda B AB Shin Jeong Eun JE Zasadzinski Joseph A JA Reich Norbert O NO

Small (Weinheim an der Bergstrasse, Germany) 20180702 30

A light-activated genome editing platform based on the release of enzymes from a plasmonic nanoparticle carrier when exposed to biocompatible near-infrared light pulses is described. The platform relies on the robust affinity of polyhistidine tags to nitrilotriacetic acid in the presence of copper which is attached to double-stranded nucleic acids self-assembled on the gold nanoparticle surface. A protein fusion of the Cre recombinase containing a TAT internalization peptide sequence to achieve ...[more]

PMID: 29968382

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