Engineered extracellular matrices facilitate brain organoids from human pluripotent stem cells.

ABSTRACT:

Objective

Brain organoids are miniaturized in vitro brain models generated from pluripotent stem cells, which resemble full-sized brain more closely than conventional two-dimensional cell cultures. Although brain organoids mimic the human brain's cell-to-cell network interactions, they generally fail to faithfully recapitulate cell-to-matrix interactions. Here, an engineered framework, called an engineered extracellular matrix (EECM), was developed to provide support and cell-to-matrix interactions to developing brain organoids.

Methods

We generated brain organoids using EECMs comprised of human fibrillar fibronectin supported by a highly porous polymer scaffold. The resultant brain organoids were characterized by immunofluorescence microscopy, transcriptomics, and proteomic

SUBMITTER: Muniz AJ 

PROVIDER: S-EPMC10351667 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature