Direct sequencing of total Saccharomyces cerevisiae tRNAs by LC-MS/MS.

ABSTRACT: Among RNAs, transfer RNAs (tRNAs) contain the widest variety of abundant posttranscriptional chemical modifications. These modifications are crucial for tRNAs to participate in protein synthesis, promoting proper tRNA structure and aminoacylation, facilitating anticodon:codon recognition, and ensuring the reading frame maintenance of the ribosome. While tRNA modifications were long thought to be stoichiometric, it is becoming increasingly apparent that these modifications can change dynamically in response to the cellular environment. The ability to broadly characterize the fluctuating tRNA modification landscape will be essential for establishing the molecular level contributions of individual sites of tRNA modification. The locations of modifications within individual tRNA sequences can

SUBMITTER: Jones JD 

PROVIDER: S-EPMC10351886 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature