Minimizing amplification bias during reverse transcription for in vitro selections.

ABSTRACT: Systematic evolution of ligands through exponential enrichment (SELEX) is widely used to identify functional nucleic acids, such as aptamers and ribozymes. Ideally, selective pressure drives the enrichment of sequences that display the function of interest (binding, catalysis, etc.). However, amplification biases from reverse transcription can overwhelm this enrichment and leave some functional sequences at a disadvantage, with cumulative effects across multiple rounds of selection. Libraries that are designed to include structural scaffolds can improve selection outcomes by sampling sequence space more strategically, but they are also susceptible to such amplification biases, particularly during reverse transcription. Therefore, we tested five reverse transcrip

SUBMITTER: Lucas JK 

PROVIDER: S-EPMC10351896 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature