Characterization of Duodenal Microbiota in Patients with Acute Pancreatitis and Healthy Controls.

ABSTRACT:

Objective

The small intestinal bacterial overgrowth (SIBO) in acute pancreatitis correlates with the severity of the disease. However, corresponding studies on the microbial composition of the duodenal mucosa of patients are uncommon.

Methods

Duodenal mucosal biopsies were collected by gastroscopy from 16 patients with mild acute pancreatitis (the Ap group) and 16 healthy individuals (the control group) and subjected to histological studies as well as bacterial 16S rRNA gene sequencing. Caerulein and L-arginine were used to induce mild acute pancreatitis (MAP) and severe acute pancreatitis (SAP) in mice, respectively, and their pancreas and duodenum were collected for histological studies.

Results

H&E analysis displayed no significant pathological damage in the descen

SUBMITTER: Zhao MQ 

PROVIDER: S-EPMC10352172 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature