The metabolic function of pyruvate kinase M2 regulates reactive oxygen species production and microbial killing by neutrophils.

ABSTRACT: Neutrophils rely predominantly on glycolytic metabolism for their biological functions, including reactive oxygen species (ROS) production. Although pyruvate kinase M2 (PKM2) is a glycolytic enzyme known to be involved in metabolic reprogramming and gene transcription in many immune cell types, its role in neutrophils remains poorly understood. Here, we report that PKM2 regulates ROS production and microbial killing by neutrophils. Zymosan-activated neutrophils showed increased cytoplasmic expression of PKM2. Pharmacological inhibition or genetic deficiency of PKM2 in neutrophils reduced ROS production and Staphylococcus aureus killing in vitro. In addition, this also resulted in phosphoenolpyruvate (PEP) accumulation and decreased dihydroxyacetone phosphate (DHAP) production, which is req

SUBMITTER: Toller-Kawahisa JE 

PROVIDER: S-EPMC10352279 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature